TY - JOUR

T1 - Role of vitamin C and E supplementation on IL-6 in response to training

AU - Yfanti, Christina

AU - Fischer, Christian P.

AU - Nielsen, Søren

AU - Åkerström, Thorbjörn

AU - Nielsen, Anders R.

AU - Veskoukis, Aristidis S.

AU - Kouretas, Demetrios

AU - Lykkesfeldt, Jens

AU - Pilegaard, Henriette

AU - Pedersen, Bente Klarlund

PY - 2012

Y1 - 2012

N2 - Vitamin C and E supplementation has been shown to attenuate the acute exercise-induced increase in plasma interleukin-6 (IL-6) concentration. Here, we studied the effect of antioxidant vitamins on the regulation of IL-6 expression in muscle and the circulation in response to acute exercise before and after high-intensity endurance exercise training. Twenty-one young healthy men were allocated into either a vitamin (VT; vitamin C and E, n = 11) or a placebo (PL, n = 10) group. A 1-h acute bicycling exercise trial at 65% of maximal power output was performed before and after 12 wk of progressive endurance exercise training. In response to training, the acute exercise-induced IL-6 response was attenuated in PL (P <0.02), but not in VT (P = 0.82). However, no clear difference between groups was observed (group × training: P = 0.13). Endurance exercise training also attenuated the acute exercise-induced increase in muscle-IL-6 mRNA in both groups. Oxidative stress, assessed by plasma protein carbonyls concentration, was overall higher in the VT compared with the PL group (group effect: P <0.005). This was accompanied by a general increase in skeletal muscle mRNA expression of antioxidative enzymes, including catalase, copper-zinc superoxide dismutase, and glutathione peroxidase 1 mRNA expression in the VT group. However, skeletal muscle protein content of catalase, copper-zinc superoxide dismutase, or glutathione peroxidase 1 was not affected by training or supplementation. In conclusion, our results indicate that, although vitamin C and E supplementation may attenuate exercise-induced increases in plasma IL-6 there is no clear additive effect when combined with endurance training.

AB - Vitamin C and E supplementation has been shown to attenuate the acute exercise-induced increase in plasma interleukin-6 (IL-6) concentration. Here, we studied the effect of antioxidant vitamins on the regulation of IL-6 expression in muscle and the circulation in response to acute exercise before and after high-intensity endurance exercise training. Twenty-one young healthy men were allocated into either a vitamin (VT; vitamin C and E, n = 11) or a placebo (PL, n = 10) group. A 1-h acute bicycling exercise trial at 65% of maximal power output was performed before and after 12 wk of progressive endurance exercise training. In response to training, the acute exercise-induced IL-6 response was attenuated in PL (P <0.02), but not in VT (P = 0.82). However, no clear difference between groups was observed (group × training: P = 0.13). Endurance exercise training also attenuated the acute exercise-induced increase in muscle-IL-6 mRNA in both groups. Oxidative stress, assessed by plasma protein carbonyls concentration, was overall higher in the VT compared with the PL group (group effect: P <0.005). This was accompanied by a general increase in skeletal muscle mRNA expression of antioxidative enzymes, including catalase, copper-zinc superoxide dismutase, and glutathione peroxidase 1 mRNA expression in the VT group. However, skeletal muscle protein content of catalase, copper-zinc superoxide dismutase, or glutathione peroxidase 1 was not affected by training or supplementation. In conclusion, our results indicate that, although vitamin C and E supplementation may attenuate exercise-induced increases in plasma IL-6 there is no clear additive effect when combined with endurance training.

KW - Adult

KW - Antioxidants

KW - Ascorbic Acid

KW - Body Mass Index

KW - Catalase

KW - Dietary Supplements

KW - Double-Blind Method

KW - Exercise

KW - Glutathione Peroxidase

KW - Humans

KW - Hydrocortisone

KW - Interleukin 1 Receptor Antagonist Protein

KW - Interleukin-6

KW - Male

KW - Muscle Proteins

KW - Muscle, Skeletal

KW - Oxidative Stress

KW - Physical Endurance

KW - Superoxide Dismutase

KW - Vitamin E

KW - vitamin C

KW - vitamin E

KW - endurance exercise

KW - interleukin-6

U2 - 10.1152/japplphysiol.01027.2010

DO - 10.1152/japplphysiol.01027.2010

M3 - Journal article

C2 - 22207723

VL - 112

SP - 990

EP - 1000

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 6

ER -