Pronounced inflammatory response to endotoxaemia during nighttime: a randomised cross-over trial
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Pronounced inflammatory response to endotoxaemia during nighttime : a randomised cross-over trial. / Alamili, Mahdi; Bendtzen, Klaus; Lykkesfeldt, Jens; Rosenberg, Jacob; Gögenur, Ismail.
In: PloS one, Vol. 9, No. 1, e87413, 2014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pronounced inflammatory response to endotoxaemia during nighttime
T2 - a randomised cross-over trial
AU - Alamili, Mahdi
AU - Bendtzen, Klaus
AU - Lykkesfeldt, Jens
AU - Rosenberg, Jacob
AU - Gögenur, Ismail
PY - 2014
Y1 - 2014
N2 - BACKGROUND: Circadian variation in bodily functions has been shown to impact health in acute and chronic medical conditions. Little is known about the relationship between circadian rhythm and sepsis in humans. We aimed to investigate circadian variations in the host response in a human endotoxaemia model.DESIGN AND METHODS: A cross-over study, where 12 healthy young men received E. coli endotoxin (lipopolysaccharide, LPS) 0.3 ng/kg at 12 noon and, on another day, at 12 midnight. Blood samples were analysed for pro- and anti-inflammatory cytokines: tumour-necrosis factor (TNF)-alpha, soluble TNF receptors (sTNF-R)-1 and -2, interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1Ra), IL-6, and IL-10 as well as YKL-40 and the oxidative stress markers malondialdehyde (MDA), ascorbic acid (AA) and dehydroascorbic acid (DHA) before and at 2, 4, 6 and 8 hours after LPS administration.RESULTS: The levels of MDA and IL-10 where significantly higher during the day time (P<0.05) whereas levels of TNF-alpha, sTNF-RI, sTNF-RII, IL-1Ra, IL-6, and YKL-40 were higher (P<0.01 for all comparisons) during the night time. No significant differences were seen in the levels of AA and DHA.CONCLUSION: A day-night difference in the acute phase response to endotoxaemia exists in healthy volunteers with a more pronounced inflammatory response during the night time. This circadian difference in the response to endotoxaemia may play an important role in the clinical setting and should be investigated further.
AB - BACKGROUND: Circadian variation in bodily functions has been shown to impact health in acute and chronic medical conditions. Little is known about the relationship between circadian rhythm and sepsis in humans. We aimed to investigate circadian variations in the host response in a human endotoxaemia model.DESIGN AND METHODS: A cross-over study, where 12 healthy young men received E. coli endotoxin (lipopolysaccharide, LPS) 0.3 ng/kg at 12 noon and, on another day, at 12 midnight. Blood samples were analysed for pro- and anti-inflammatory cytokines: tumour-necrosis factor (TNF)-alpha, soluble TNF receptors (sTNF-R)-1 and -2, interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1Ra), IL-6, and IL-10 as well as YKL-40 and the oxidative stress markers malondialdehyde (MDA), ascorbic acid (AA) and dehydroascorbic acid (DHA) before and at 2, 4, 6 and 8 hours after LPS administration.RESULTS: The levels of MDA and IL-10 where significantly higher during the day time (P<0.05) whereas levels of TNF-alpha, sTNF-RI, sTNF-RII, IL-1Ra, IL-6, and YKL-40 were higher (P<0.01 for all comparisons) during the night time. No significant differences were seen in the levels of AA and DHA.CONCLUSION: A day-night difference in the acute phase response to endotoxaemia exists in healthy volunteers with a more pronounced inflammatory response during the night time. This circadian difference in the response to endotoxaemia may play an important role in the clinical setting and should be investigated further.
KW - Adipokines
KW - Analysis of Variance
KW - Circadian Rhythm
KW - Cross-Over Studies
KW - Cytokines
KW - Endotoxemia
KW - Humans
KW - Inflammation
KW - Interleukin 1 Receptor Antagonist Protein
KW - Interleukin-10
KW - Interleukin-1beta
KW - Interleukin-6
KW - Lectins
KW - Lipopolysaccharides
KW - Male
KW - Malondialdehyde
KW - Oxidative Stress
KW - Receptors, Tumor Necrosis Factor
KW - Time Factors
KW - Tumor Necrosis Factor-alpha
U2 - 10.1371/journal.pone.0087413
DO - 10.1371/journal.pone.0087413
M3 - Journal article
C2 - 24475284
VL - 9
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e87413
ER -
ID: 124427290