Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance. / Jothery, Aqeel H. Al; Vaanholt, Lobke M.; Mody, Nimesh; Arnous, Anis; Lykkesfeldt, Jens; Bunger, Lutz; Hill, William G.; Mitchell, Sharon E.; Allison, David B.; Speakman, John R.
In: Scientific Reports, Vol. 6, 36353 , 14.11.2016.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance
AU - Jothery, Aqeel H. Al
AU - Vaanholt, Lobke M.
AU - Mody, Nimesh
AU - Arnous, Anis
AU - Lykkesfeldt, Jens
AU - Bunger, Lutz
AU - Hill, William G.
AU - Mitchell, Sharon E.
AU - Allison, David B.
AU - Speakman, John R.
PY - 2016/11/14
Y1 - 2016/11/14
N2 - Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.
AB - Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.
U2 - 10.1038/srep36353
DO - 10.1038/srep36353
M3 - Journal article
C2 - 27841266
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 36353
ER -
ID: 169437371