Kinetics of acid-induced degradation of tetra- and dihydrobiopterin in relation to their relevance as biomarkers of endothelial function
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Kinetics of acid-induced degradation of tetra- and dihydrobiopterin in relation to their relevance as biomarkers of endothelial function. / Mortensen, Alan; Lykkesfeldt, Jens.
In: Biomarkers, Vol. 18, No. 1, 2013, p. 55-62.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Kinetics of acid-induced degradation of tetra- and dihydrobiopterin in relation to their relevance as biomarkers of endothelial function
AU - Mortensen, Alan
AU - Lykkesfeldt, Jens
PY - 2013
Y1 - 2013
N2 - The ratio of the nitric oxide synthase (NOS) cofactor tetrahydrobiopterin (BH(4)) to its oxidized form dihydrobiopterin (BH(2)) has been suggested as an index of endothelial dysfunction. Consequently, much effort has been put into preserving the in vivo equilibrium between these labile analytes. In the present study, we conducted a series of stability experiments in aqueous solutions and blood to identify the most appropriate way of stabilizing BH(4) and BH(2). Based on our results, we are able to recommend that blood samples are immediately stabilized with dithioerythriol and protein precipitation conducted using trichloroacetic acid (TCA).
AB - The ratio of the nitric oxide synthase (NOS) cofactor tetrahydrobiopterin (BH(4)) to its oxidized form dihydrobiopterin (BH(2)) has been suggested as an index of endothelial dysfunction. Consequently, much effort has been put into preserving the in vivo equilibrium between these labile analytes. In the present study, we conducted a series of stability experiments in aqueous solutions and blood to identify the most appropriate way of stabilizing BH(4) and BH(2). Based on our results, we are able to recommend that blood samples are immediately stabilized with dithioerythriol and protein precipitation conducted using trichloroacetic acid (TCA).
U2 - 10.3109/1354750X.2012.730552
DO - 10.3109/1354750X.2012.730552
M3 - Journal article
C2 - 23066920
VL - 18
SP - 55
EP - 62
JO - Biomarkers
JF - Biomarkers
SN - 1354-750X
IS - 1
ER -
ID: 43475489