Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture

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Standard

Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture. / Frikke-Schmidt, Henriette Rønne; Lykkesfeldt, Jens.

In: Analytical Biochemistry, Vol. 397, No. 1, 2010, p. 135-137.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Frikke-Schmidt, HR & Lykkesfeldt, J 2010, 'Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture', Analytical Biochemistry, vol. 397, no. 1, pp. 135-137. https://doi.org/10.1016/j.ab.2009.09.040

APA

Frikke-Schmidt, H. R., & Lykkesfeldt, J. (2010). Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture. Analytical Biochemistry, 397(1), 135-137. https://doi.org/10.1016/j.ab.2009.09.040

Vancouver

Frikke-Schmidt HR, Lykkesfeldt J. Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture. Analytical Biochemistry. 2010;397(1):135-137. https://doi.org/10.1016/j.ab.2009.09.040

Author

Frikke-Schmidt, Henriette Rønne ; Lykkesfeldt, Jens. / Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture. In: Analytical Biochemistry. 2010 ; Vol. 397, No. 1. pp. 135-137.

Bibtex

@article{bba5eb40a8df11debc73000ea68e967b,
title = "Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture",
abstract = "It is generally accepted that the addition of vitamin C to cell culture medium improves cell growth. However, once added, the vitamin C concentration declines rapidly. This situation differs from the in vivo environment where the endothelium is constantly supplied with ascorbate from the blood. With a focus on intracellular vitamin C, we simulated constant supply of ascorbate by the hourly addition of freshly prepared medium containing 75 lM ascorbate and subsequently compared it with more practical regimens using combinations of ascorbate and 2-phosphoascorbate. We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h.",
author = "Frikke-Schmidt, {Henriette R{\o}nne} and Jens Lykkesfeldt",
year = "2010",
doi = "10.1016/j.ab.2009.09.040",
language = "English",
volume = "397",
pages = "135--137",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture

AU - Frikke-Schmidt, Henriette Rønne

AU - Lykkesfeldt, Jens

PY - 2010

Y1 - 2010

N2 - It is generally accepted that the addition of vitamin C to cell culture medium improves cell growth. However, once added, the vitamin C concentration declines rapidly. This situation differs from the in vivo environment where the endothelium is constantly supplied with ascorbate from the blood. With a focus on intracellular vitamin C, we simulated constant supply of ascorbate by the hourly addition of freshly prepared medium containing 75 lM ascorbate and subsequently compared it with more practical regimens using combinations of ascorbate and 2-phosphoascorbate. We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h.

AB - It is generally accepted that the addition of vitamin C to cell culture medium improves cell growth. However, once added, the vitamin C concentration declines rapidly. This situation differs from the in vivo environment where the endothelium is constantly supplied with ascorbate from the blood. With a focus on intracellular vitamin C, we simulated constant supply of ascorbate by the hourly addition of freshly prepared medium containing 75 lM ascorbate and subsequently compared it with more practical regimens using combinations of ascorbate and 2-phosphoascorbate. We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h.

U2 - 10.1016/j.ab.2009.09.040

DO - 10.1016/j.ab.2009.09.040

M3 - Journal article

C2 - 19782654

VL - 397

SP - 135

EP - 137

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

ID: 14664819