Distribution of vitamin C is tissue specific with early saturation of the brain and adrenal glands following differential oral dose regimens in guinea pigs
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Distribution of vitamin C is tissue specific with early saturation of the brain and adrenal glands following differential oral dose regimens in guinea pigs. / Andersen, Stine Hasselholt; Tveden-Nyborg, Pernille; Lykkesfeldt, Jens.
In: British Journal of Nutrition, Vol. 113, No. 10, 2015, p. 1539-1549.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Distribution of vitamin C is tissue specific with early saturation of the brain and adrenal glands following differential oral dose regimens in guinea pigs
AU - Andersen, Stine Hasselholt
AU - Tveden-Nyborg, Pernille
AU - Lykkesfeldt, Jens
PY - 2015
Y1 - 2015
N2 - Vitamin C (VitC) deficiency is surprisingly common in humans even in developed parts of the world. The micronutrient has several established functions in the brain; however, the consequences of its deficiency are not well characterised. To elucidate the effects of VitC deficiency on the brain, increased knowledge about the distribution of VitC to the brain and within different brain regions after varying dietary concentrations is needed. In the present study, guinea pigs (like humans lacking the ability to synthesise VitC) were randomly divided into six groups (n 10) that received different concentrations of VitC ranging from 100 to 1500 mg/kg feed for 8 weeks, after which VitC concentrations in biological fluids and tissues were measured using HPLC. The distribution of VitC was found to be dynamic and dependent on dietary availability. Brain saturation was region specific, occurred at low dietary doses, and the dose-concentration relationship could be approximated with a three-parameter Hill equation. The correlation between plasma and brain concentrations of VitC was moderate compared with other organs, and during non-scorbutic VitC deficiency, the brain was able to maintain concentrations from about one-quarter to half of sufficient levels depending on the region, whereas concentrations in other tissues decreased to one-sixth or less. The adrenal glands have similar characteristics to the brain. The observed distribution kinetics with a low dietary dose needed for saturation and exceptional retention ability suggest that the brain and adrenal glands are high priority tissues with regard to the distribution of VitC.
AB - Vitamin C (VitC) deficiency is surprisingly common in humans even in developed parts of the world. The micronutrient has several established functions in the brain; however, the consequences of its deficiency are not well characterised. To elucidate the effects of VitC deficiency on the brain, increased knowledge about the distribution of VitC to the brain and within different brain regions after varying dietary concentrations is needed. In the present study, guinea pigs (like humans lacking the ability to synthesise VitC) were randomly divided into six groups (n 10) that received different concentrations of VitC ranging from 100 to 1500 mg/kg feed for 8 weeks, after which VitC concentrations in biological fluids and tissues were measured using HPLC. The distribution of VitC was found to be dynamic and dependent on dietary availability. Brain saturation was region specific, occurred at low dietary doses, and the dose-concentration relationship could be approximated with a three-parameter Hill equation. The correlation between plasma and brain concentrations of VitC was moderate compared with other organs, and during non-scorbutic VitC deficiency, the brain was able to maintain concentrations from about one-quarter to half of sufficient levels depending on the region, whereas concentrations in other tissues decreased to one-sixth or less. The adrenal glands have similar characteristics to the brain. The observed distribution kinetics with a low dietary dose needed for saturation and exceptional retention ability suggest that the brain and adrenal glands are high priority tissues with regard to the distribution of VitC.
KW - Adrenal Glands
KW - Animals
KW - Animals, Outbred Strains
KW - Ascorbic Acid
KW - Ascorbic Acid Deficiency
KW - Brain
KW - Cerebellum
KW - Dietary Supplements
KW - Female
KW - Frontal Lobe
KW - Guinea Pigs
KW - Hippocampus
KW - Kidney
KW - Kinetics
KW - Liver
KW - Neurons
KW - Organ Specificity
KW - Phosphorylation
KW - Random Allocation
KW - Tissue Distribution
U2 - 10.1017/S0007114515000690
DO - 10.1017/S0007114515000690
M3 - Journal article
C2 - 25865869
VL - 113
SP - 1539
EP - 1549
JO - British Journal of Nutrition
JF - British Journal of Nutrition
SN - 0007-1145
IS - 10
ER -
ID: 144253621