An ELISA for detection of complement-bound circulating immune complexes in mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An ELISA for detection of complement-bound circulating immune complexes in mice. / Boysen, Lykke; Lauritzen, Brian; Viuff, Birgitte Martine; Lykkesfeldt, Jens; Landsy, Lone Hummelshøj.

In: Journal of Immunotoxicology, Vol. 16, No. 1, 2019, p. 82-86.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boysen, L, Lauritzen, B, Viuff, BM, Lykkesfeldt, J & Landsy, LH 2019, 'An ELISA for detection of complement-bound circulating immune complexes in mice', Journal of Immunotoxicology, vol. 16, no. 1, pp. 82-86. https://doi.org/10.1080/1547691X.2019.1599471

APA

Boysen, L., Lauritzen, B., Viuff, B. M., Lykkesfeldt, J., & Landsy, L. H. (2019). An ELISA for detection of complement-bound circulating immune complexes in mice. Journal of Immunotoxicology, 16(1), 82-86. https://doi.org/10.1080/1547691X.2019.1599471

Vancouver

Boysen L, Lauritzen B, Viuff BM, Lykkesfeldt J, Landsy LH. An ELISA for detection of complement-bound circulating immune complexes in mice. Journal of Immunotoxicology. 2019;16(1):82-86. https://doi.org/10.1080/1547691X.2019.1599471

Author

Boysen, Lykke ; Lauritzen, Brian ; Viuff, Birgitte Martine ; Lykkesfeldt, Jens ; Landsy, Lone Hummelshøj. / An ELISA for detection of complement-bound circulating immune complexes in mice. In: Journal of Immunotoxicology. 2019 ; Vol. 16, No. 1. pp. 82-86.

Bibtex

@article{d6bf4c15f0a04895a1f8b4ee5f241523,
title = "An ELISA for detection of complement-bound circulating immune complexes in mice",
abstract = "Measurements of complement-bound circulating immune complexes (cCICs) in pre-clinical studies may provide important information about the etiology of certain pathology findings suggestive of being immune complex mediated. This article describes the development and qualification of a universal methodology to measure cCIC in mice after dosing with species foreign proteins. The assay is a sandwich enzyme-linked immunosorbent assay–exclusively based on commercially available reagents–that could detect mouse IgG bound to complement C3 independent of the test-substance present in the plasma sample. Heat-aggregated serum was used as positive control. The assay was qualified by assessment of acceptance criteria, stability of positive control, precision, and specificity. Finally, the performance of the assay was tested using plasma from mice administered either of three different proteins, i.e bovine serum albumin (BSA), a fully human monoclonal antibody, and a humanized monoclonal antibody.",
keywords = "complement 3 (C3), immune complex, Immunoassay, immunogenicity",
author = "Lykke Boysen and Brian Lauritzen and Viuff, {Birgitte Martine} and Jens Lykkesfeldt and Landsy, {Lone Hummelsh{\o}j}",
year = "2019",
doi = "10.1080/1547691X.2019.1599471",
language = "English",
volume = "16",
pages = "82--86",
journal = "Journal of Immunotoxicology",
issn = "1547-691X",
publisher = "Taylor & Francis",
number = "1",

}

RIS

TY - JOUR

T1 - An ELISA for detection of complement-bound circulating immune complexes in mice

AU - Boysen, Lykke

AU - Lauritzen, Brian

AU - Viuff, Birgitte Martine

AU - Lykkesfeldt, Jens

AU - Landsy, Lone Hummelshøj

PY - 2019

Y1 - 2019

N2 - Measurements of complement-bound circulating immune complexes (cCICs) in pre-clinical studies may provide important information about the etiology of certain pathology findings suggestive of being immune complex mediated. This article describes the development and qualification of a universal methodology to measure cCIC in mice after dosing with species foreign proteins. The assay is a sandwich enzyme-linked immunosorbent assay–exclusively based on commercially available reagents–that could detect mouse IgG bound to complement C3 independent of the test-substance present in the plasma sample. Heat-aggregated serum was used as positive control. The assay was qualified by assessment of acceptance criteria, stability of positive control, precision, and specificity. Finally, the performance of the assay was tested using plasma from mice administered either of three different proteins, i.e bovine serum albumin (BSA), a fully human monoclonal antibody, and a humanized monoclonal antibody.

AB - Measurements of complement-bound circulating immune complexes (cCICs) in pre-clinical studies may provide important information about the etiology of certain pathology findings suggestive of being immune complex mediated. This article describes the development and qualification of a universal methodology to measure cCIC in mice after dosing with species foreign proteins. The assay is a sandwich enzyme-linked immunosorbent assay–exclusively based on commercially available reagents–that could detect mouse IgG bound to complement C3 independent of the test-substance present in the plasma sample. Heat-aggregated serum was used as positive control. The assay was qualified by assessment of acceptance criteria, stability of positive control, precision, and specificity. Finally, the performance of the assay was tested using plasma from mice administered either of three different proteins, i.e bovine serum albumin (BSA), a fully human monoclonal antibody, and a humanized monoclonal antibody.

KW - complement 3 (C3)

KW - immune complex

KW - Immunoassay

KW - immunogenicity

U2 - 10.1080/1547691X.2019.1599471

DO - 10.1080/1547691X.2019.1599471

M3 - Journal article

C2 - 31271074

AN - SCOPUS:85069267398

VL - 16

SP - 82

EP - 86

JO - Journal of Immunotoxicology

JF - Journal of Immunotoxicology

SN - 1547-691X

IS - 1

ER -

ID: 236214757